Bamboo is a plant with a variety of uses and ecological values, but due to the low efficiency of germplasm innovation such as hybrid breeding and genetic transformation, making the slow process of variety selection, which hinders its oriented-improvement. In response to the practical demand for improved varieties in bamboo industry, Prof. Yuan Jinling carries out genetic improvement researches on bamboo from the aspects of germplasm innovation technology, genetic mechanism analysis and variety selection practice. Under the funding of several national, provincial and other-resource projects, she has constructed some distant cross-breeding techniques, somatic embryogenesis and genetic transformation techniques, as well as some regulations for new variety selection and high-efficiency cultivation of bamboos.
By conducting nationwide investigation on bamboo resources and transplanting protection, Prof. Yuan Jinling and her team reported some new taxa and new records of Bambusoideae, and live bamboo resource libraries have been established in several provinces of Zhejiang, Anhui, Fujian. Through comprehensive comparation and practical verification of bamboo characteristics, we have made a guideline for the conduct of tests for distinctness, uniformity and stability (DUS) - Phyllostachy Siebold et Zuccarini. After years of investigation and field experiment on bamboo flowering habits, they established the crossing pollination techniques of Bambusa multiplex × Dendrocalamus latiflorus, B. multiplex × B. chungii, etc. Extensive morphological variations were observed within hybrid populations on growth, morphology, and fiber, and growth advantages, intermediate values between parental lines, strong maternal and paternal effects were found in the progenies. Also, they selected some hybrids with good fiber-quality for reproduction and afforestation. They has conducted bamboo tissue culture for over 20 years, by using the seed embryo as explants, we induced embryogenic callus from B. multiplex, B. chungii, Ph. edulis, Ph. Irridescens, etc, and developed somatic embryogenesis. Based on those tissue culture techniques, we constructed agrobacterium-mediated transformation protocols, and they will isolate some genes from bamboo and conduct transformation validation in the following.
Efficient plant regeneration and genetic transformation system construction of bamboo, 2021-2026
Genetic regulation of main traits and molecular assisted breeding of Shoot-oriented bamboo, 2021-2025
Demonstration of bamboo industry upgrading techniques in Longsheng based on variety selection and cultivation, 2023-2025
Revision on a bamboo industrial standard-Technical regulations for cultivation of sympodial bamboo stands, 2023-2025
Research on breeding technologies and elite resource selection on important economic bamboos, 2016-2020
Establishment of protocol for genetic transformation in Phyllostachys edulis based on somatic embryogenesis, 2016-2018
Guidelines for the conduct of tests for distinctness, uniformity and stability (DUS) ——Phyllostachy Siebold et Zuccarini, 2016-2018
Establishment of bamboo cell engineering breeding technology platform, 2014-2017
Creation of high-quality and resistant new bamboo germplasm by genetic transformation, 2009-2011
Introduction of key technologies for germplasm innovation of bamboo,2011-2013
Yu L, Yue J, Dai Y, Zhang L, Wang Q, Yuan J. Characterization of color variation in bamboo sheath of Chimonobambusa hejiangensis by UPLC-ESI-MS/MS and RNA sequencing. BMC Plant Biol. 2023, 23(1):466.
Yuan J, Yue J, Ma J, Yu L, Liu L. Culm Form Characteristics of Phyllostachys edulis 'Yuanbao'. Scientia Silvae Sinicae, 2023, 59(05):71-80. doi:10.11707/j.1001-7488. LYKX20220403 (in Chinese)
Yu L, Yue J, Huang W, Ma M, Zhang L, Wang Q, Yuan J. Embryonic callus induction and genetic transformation of Chimonobambusa quadrangularis. Plant Physiology Journal 2022, 58 (11): 2099-2106 (in Chinese)
Yuan JL, Ma JX, Zhong YB, et al. SSR-based hybrid identification, genetic analyses and fingerprint development of hybridization progenies from sympodial bamboo (Bambusoideae, Poaceae). Journal of Nanjing Forestry University (Natural Science Edition), 2021, 45(5): 10-18.DOI: 10.12302/j.issn.1000-2006.202012046.
Yue J-J, Yuan J-L, Wu F-H, Yuan Y-H, Cheng Q-W, Hsu C-T and Lin C-S (2021). Protoplasts: From Isolation to CRISPR/Cas Genome Editing Application. Front. Genome Ed. 3 (19), 717017. doi:10.3389/fgeed.2021.717017
Yuan, JL., Yue, JJ., Zhong, YB. et al. Genetic variation in distant and inbred hybridization progenies from three sympodial bamboo parent species. J. For. Res. 30, 1323–1329 (2019)
Yuan JL, Yue JJ, Gu XP, et al. Flowering of woody bamboo in tissue culture systems [J]. Frontiers in plant science, 8:1589. doi: 10.3389/fpls.2017.01589, 2017
Zhong YB, Yue JJ, Lou C, Yuan JJ, Gu XP. Floral organ and breeding system of Dendrocalamus latiflorus. Scientia Silvae Sinicae, 2017, 53(1):1-10 (in Chinese)
Yuan JL, Sun HM, Guo GP, et al. Correlation between DNA methylation and chronological age of Moso bamboo (Phyllostachys heterocycla var. pubescens). Botanical studies, 55(4), doi:10.1186/1999-3110-55-4, 2014
Shih MC, Chou ML, Yue JJ, Hsu CT, Chang WJ, Ko SS, Liao DC, Huang YT, Chen JJ, Yuan JL, Gu XP, Lin CS. BeMADS1 is a key to delivery MADSs into nucleus in reproductive tissues-De novo characterization of Bambusa edulis transcriptome and study of MADS genes in bamboo floral development [J]. BMC plant biology, 14:179, doi: 10.1186/1471-2229-14-179, 2014
Yuan JL, Yue JJ, Wu XL, et al. Protocol for callus induction and somatic embryogenesis in Moso bamboo [J]. PLoS ONE, 8(12): e81954, doi:10.1371/journal. pone.0081954, 2013
Yuan JL, Gu XP, Yue JJ, Ma NX, Chen YT. Flowering Biology and Crossing of Bambusa multiplex. Scientia Silvae Sinicae, 2011, 47(8):61-66 (in Chinese)
Ma NX, Zhang WY, Yuan JL, Yue JJ. Flora of Zhejiang (New Edition), Volume 9, Poaceae, Bambusoideae, Hangzhou: Zhejiang Science and Technology Press, 2021
Fu JH, Zhang WY, Yuan JL. Chinese Giant Pandas and their edible bamboos. Hangzhou: Zhejiang Science and Technology Press, 2011
Ma NX, Zhang WY. Endemic and rare bamboos in China. Hangzhou: Zhejiang Science and Technology Press, 2007
National approved bamboo variety, Phyllostachys vivax 'Huanggan', S-SV-PV-004-2022, 2022
National approved bamboo variety, Phyllostachys edulis 'Yuanbao', S-SV-PED-005-2022, 2022
Patent. A method for bamboo's crossing pollination, 2019, ZL201710615389.4
Patent. A method for protoplast culture on Moso bamboo (Phyllostachys edulis), 2017, ZL201510566124.0
Patent. A method for endosperm culture on Moso bamboo (Phyllostachys edulis), 2017, ZL201510566151.8
Patent. A method for somatic embryogenesis on Moso bamboo (Phyllostachys edulis), 2013, ZL201210162457.3
Patent. A method for plantlet regeneration on Bambusa multiples, 2012, ZL200910096294.1.
